The village is an important inheritance of urban function diffusion and transfer, and has unique functions such as ecological landscape function, agricultural economic function, social emotional function, cultural aesthetics and so on. In the process of urbanization in China, the development concept of ‘focusing on city, ignoring rural’ has sacrificed the interests of the countryside for a long time. The development path of economic efficiency has neglected the protection of rural multi-value functions such as ecology, society and culture, and how to understand the characteristics and laws of the regional differentiation of rural functions in the new period, how to integrate the population, industry, infrastructure and ecological environment between urban and rural areas, and how to solve the problem of regional differentiation and functions. Constructing the functional structure system of organic coordination between urban and rural, so that the promotion of rural functions not only can become an important fulcrum to crack the "urban disease" and "rural disease", but also gradually develop the village into an unique charm and competitively happy home, and it has become an important issue to be solved in the theoretical research, urban and rural integrated development and beautiful rural construction practice. Based on the index system and measure model of rural function evaluation, this study takes the 23 counties and districts of Changsha-Zhuzhou-Xiangtan urban agglomeration area as the regional unit, and analyzes the regional differentiation characteristics of rural economic development, grain production, social security and eco-tourism in the study area. The results show that: the economic development function, grain production function, social security function and eco-tourism function of Changsha-Zhuzhou-Xiangtan urban agglomeration are generally low, but the development of each function is more balanced; the regional characteristics of rural function of Chang-Zhu-Tan urban agglomeration is obvious, the economic-oriented function areas are mainly distributed in the core area of Changsha-Zhuzhou-Xiangtan urban agglomeration, mainly in 6 districts of Changsha City and Changsha County, the seat of the National Economic and Technological Development Zones, and the grain production-oriented function areas are mainly distributed in Lusong District, Liuyang City, Xiangtan County, Ningxiang County, the social security function shows a gradual decrease from the central city to the periphery, and the farther away from the central city, the weaker the function, the strong distributions of eco-tourism functions are mainly in mountain and hilly areas of Chaling County , Furong District, Yuelu District, Shaoshan City, Yanling County, Ningxiang County, Liuyang City, Yuhua District and so on .