Abstract: This paper aims at supplying a scientific basis for uniting the normal reference value standard of hemoglobin of Chinese presenile men,a research is made about relationship between the reference value of Chinese healthy presenile men's hemoglobin and five geographical factors in 161 areas in China, the normal reference value is determined by the hemoglobincyanide method. The ages of the volunteers range from 46 to 59 years old. It is found that the correlation of geographical factors and the reference value of Chinese presenile men's hemoglobin are quite significant (F=192.81) from the following equation:
Ŷ=150.7+0.005 880 X₁-1.452 X₄+0.0113 1 X₅±17.9
F=192.81
In the above equation, is the normal reference value of presenile men's hemoglobin (g/L); X₁ is the altitude (m); X₄ is the annual average temperature (℃); X₅ is the annual precipitation (mm); 17.9 are the value of the 1.96 residual standard deviations. If geographical values are obtained in some areas, the reference value of Chinese presenile men's hemoglobin of this area can be reckoned using regression equation. For example, in the Beijing area, the altitude is 31.2 m, the annual average temperature is 11.5 ℃, and the annual precipitation is 644.2 mm. By means of the regression equation, the following can be calculated:
Ŷ=150.7+0.005880×31.2-1.452×11.5+0.01131×644.2±17.9=141.5±17.9
So, the calculated normal reference value of presenile men's hemoglobin can be obtained, the normal reference value of presenile men's hemoglobin is 141.5±17.9 g/L. Furthermore, according to the similarity of the reference value of Chinese presenile men's hemoglobin, taking the altitude as the main dividing basis and considering effects of other geographical factors and population distribution, China can be divided into six regions: Qinghai-Xizang, Southwest, Northwest, Southeast, North and Northeast regions.