地理科学 ›› 2007, Vol. 27 ›› Issue (6): 849-852.doi: 10.13249/j.cnki.sgs.2007.06.849

• 研究报道 • 上一篇    下一篇

砾间接触氧化反应器中细菌多样性评价

林山杉1, 金玉花2, 付丽丽3   

  1. 1. 东北师范大学城市与环境科学学院, 吉林, 长春, 130024;
    2. 中国石油集团工程设计有限责任公司东北分公司, 吉林, 132002;
    3. 辽宁石油化工大学储运与建筑工程学院建筑工程系, 辽宁, 抚顺, 113001
  • 收稿日期:2006-11-26 修回日期:2007-04-17 出版日期:2007-11-20 发布日期:2007-11-20
  • 基金资助:
    国家自然科学基金(30670059);吉林省科技厅项目(20040702-1)资助

Phylogenetic Diversity Assessment of Bacteria In a Gravel Contact Oxidation Reactor

LIN Shan-Shan1, JIN Yu-Hua2, FU Li-li3   

  1. 1. School of City and Environmental Science, Northeast Normal University, Changchun Jilin 130024;
    2. China Petroleum Group Engineering Design Co.Ltd., Jilin division, Jilin 132002;
    3. School of Storage &Architecture Engineering, Liaoning University of Petroleum and Chemical Technology, Fushun Liaoning 113001
  • Received:2006-11-26 Revised:2007-04-17 Online:2007-11-20 Published:2007-11-20

摘要: 采用分子生物学手段,通过构建16S rDNA基因文库,对新型剩余污泥减量化处理系统——砾间接触氧化反应器中细菌多样性进行了系统发育分析;通过多样性指数、丰富度指数、均匀度指数及优势度指数,对细菌多样性及优势菌种富集状况进行了系统评价。结果表明:共有80个克隆子,分别代表29个OTU,用于系统细菌分析。其中,石球外膜46个克隆子(17个OTU),石球内泥34个克隆子(12个OTU)。生物膜中细菌多样性及优势菌种富集状况均好于孔隙内泥中的细菌。

Abstract: The phylogenetic diversities of bacteria in a gravel contact oxidation reactor,a novel equipment for excess sludge decrement,were studied by constructed 16S rDNA library,PCR-based molecular surveys of environmental 16S rDNA genes.Total 80 16S rDNA gene clones were retrieved and evaluated their phylogenetic diversity.46 clones(17 OTUs) were isolated from the biofilm,and 34 clones(12 OTUs) were from the inner sludge.The measured bacteria diversities were evaluated by diversity indices,richness indices,evenness indices and dominance indices.The evaluation results indicated that both diversity and dominance of the bacteria isolated from biofilm were higher than inner sludge.

中图分类号: 

  • X131.2