黄河三角洲石油污染对湿地芦苇和碱蓬幼苗生长影响的模拟研究

doi:10.13249/j.cnki.sgs.2012.010.1254

地理科学 ›› 2012, Vol. 32 ›› Issue (10): 1254-1261.doi: 10.13249/j.cnki.sgs.2012.010.1254

黄河三角洲石油污染对湿地芦苇和碱蓬幼苗生长影响的模拟研究

于君宝¹(), 阚兴艳^1,², 王雪宏^1,², 韩广轩¹, 管博¹, 谢文军³, 林乾新⁴

1. 中国科学院烟台海岸带研究所,中国科学院海岸带环境过程重点实验室,山东省海岸带环境过程重点实验室,山东烟台 264003
2. 中国科学院研究生院, 北京 100049
3. 滨州学院山东省黄河三角洲生态环境重点实验室,山东滨州 256600
4. Department of Oceanography and Coastal Sciences, Louisiana State University, Baton Rouge, LA 70803, USA

收稿日期:2011-09-15 修回日期:2011-11-23 出版日期:2012-10-20 发布日期:2012-10-20
作者简介:
作者简介：于君宝(1970-),吉林长春人,博士,研究员,主要从事湿地生物地球化学与生态修复研究。E-mail：Junbao.yu@gmail.com
基金资助:
中国科学院-国家外国专家局创新团队国际合作伙伴计划、山东省自然科学杰出青年基金项目（JQ201114）、中国科学院知识创新工程重要方向项目（KZCX2-YW-223;KZCX2-YW-359）、中国科学院百人计划项目和十二五”国家科技支撑计划项目(2011BAC02B01)

Simulative Study on Effects of Petroleum Contamination on Seedling Growth of Phragmites australis and Suaeda salsa in Coastal Wetland of Yellow River Delta

Jun-bao YU¹(), Xing-yan KAN^1,², Xue-hong WANG^1,², Guang-xuan HAN¹, Bo GUAN¹, Wen-jun XIE³, Qian-xin LIN⁴

1. Key Laboratory of Coastal Zone Environmental Processes, Shandong Provincial Key Laboratory of Coastal Zone Environmental Processes, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences,Yantai,Shandong 264003, P. R. China
2. Graduate University of Chinese Academy of Sciences, Beijing 100049, China
3. Shandong Provincial Key Laboratory of Ecology and Environment of Yellow River Delta, Binzhou University, Binzhou 256603, China
4. Department of Oceanography and Coastal Sciences, Louisiana State University, Baton Rouge, LA 70803, USA

Received:2011-09-15 Revised:2011-11-23 Online:2012-10-20 Published:2012-10-20

摘要/Abstract

摘要：

黄河三角洲湿地是中国最具代表性的滨海湿地之一,也是中国第二大油田——胜利油田的产油区,石油污染已成为本区湿地生态系统退化的重要因子。在温室条件下模拟研究了该区滨海湿地土壤中2种不同浓度(0.5%、2.0%)石油污染对黄河三角洲地区典型物种芦苇（Phragmites australis）和盐地碱蓬(Suaeda salsa)幼苗生长的影响。结果表明,随着石油浓度的增高,芦苇和盐地碱蓬株高的受抑制程度不断增强,随着时间的推移,石油污染对芦苇分蘖的抑制作用减弱,而对盐地碱蓬幼苗的分枝数抑制作用加大。随石油浓度的增加,盐地碱蓬幼苗叶片中可溶性蛋白质含量先升高后降低,丙二醛含量和过氧化物酶活力先降低后升高,过氧化氢酶活力显著降低;芦苇幼苗则对石油污染表现出相对较强的耐受性。不同石油污染条件下芦苇幼苗叶片叶绿素含量表现出先下降后升高的变化趋势。从整体上来看,石油烃类污染对盐地碱蓬幼苗的抑制作用要高于芦苇幼苗,也说明在处理石油烃类污染物中芦苇比碱蓬更具有优势。

关键词: 黄河三角洲湿地, 石油污染, 芦苇, 盐地碱蓬, 模拟研究

Abstract:

The Yellow River Delta is a typical and representative coastal wetland in China. As the Shengli Oil Field, which is the second largest oil field in China, locates in the region, the crude oil contamination has become a key reason which causes deterioration of coastal wetland ecological system in this area. A simulative study was conducted to explain the effects of different concentration of petroleum (0.5%、2.0%) contaminated wetland soils on growth of reed（Phragmites australis）and seepweed (Suaeda salsa) seedling in the greenhouse. The soil samples without oil pollution were collected in coastal wetlands in the Yellow River Delta of soils in May 2010. The soil oil pollution levels was controlled by three oil concentration (0%, 0.5%, 2.0%) in the simulative experiment in greenhouse, four replicates for each treatment. The crude oil in experiments collected from the Shengli Oil Field production wells. The typical representative plants of reed and seepweed in coastal wetlands in the Yellow River Delta were cultivated in the different soil treatments, respectively. The reed was planted with root with same buds and the seepweed seed was sowed (0.5 g per pot). During experiment period, the seedling height of both reed and seepweeed, tillering number of reed, leaf number and branch number of seepweed were recorded. The soluble protein content, Catalase activity (CAT) activities, peroxidase activity (POD) activities, malondialdehyde (MDA) content, Chlorophyll (Chl) content of seedling leaves were determined, then the ratio of chl-a/ chl-b was calculated. The results showed that, with increasing petroleum concentration, the inhibitory effects of petroleum for plant height of reed and seepweed were increased. The inhibitory effects of petroleum contamination on reed tillering number were weakened with time, while that functions on seepweed branch number were strengthened. With increasing oil concentration in soils, the soluble protein content in seedling leaves of seepweed was decline after increase, while the opposite regulars were observed for MDA content and POD activities, and the CAT activities was decreased obviously. Compared to seepweed seedling, the reed seedling appeared high oil contamination tolerance. The Chl content in reed seedling leaves shows a trend of increase after decline with time under different condition of oil contamination. In generally, the inhibitory effects of soil oil contamination for seepweed seedling were higher than that for reed seedling. It indicated that the reed was much more suitable for restoration of oil contaminated soil than seepweed. Our results suggested that both reed and seepweed with high salt-tolerance properties could be extensively applied in soil restoration of oil contamination regions in the coastal wetland of Yellow River Delta.

Key words: wetland of Yellow River Delta, petroleum contamination, Phragmites australis, Suaeda salsa, simulative study

中图分类号:

X142

于君宝, 阚兴艳, 王雪宏, 韩广轩, 管博, 谢文军, 林乾新. 黄河三角洲石油污染对湿地芦苇和碱蓬幼苗生长影响的模拟研究[J]. 地理科学, 2012, 32(10): 1254-1261.

Jun-bao YU, Xing-yan KAN, Xue-hong WANG, Guang-xuan HAN, Bo GUAN, Wen-jun XIE, Qian-xin LIN. Simulative Study on Effects of Petroleum Contamination on Seedling Growth of Phragmites australis and Suaeda salsa in Coastal Wetland of Yellow River Delta[J]. SCIENTIA GEOGRAPHICA SINICA, 2012, 32(10): 1254-1261.

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参考文献 33

[1]	陶思明. 黄河三角洲湿地生态与石油生产:保护、冲突和协调发展[J]. 环境保护, 2000, 6: 26~28.
[2]	余萃, 廖先清, 刘子国, 等. 石油污染土壤的微生物修复研究进展[J]. 湖北农业科技, 2009, 48(5): 1260~1263.
[3]	徐东霞, 章光新. 人类活动对中国滨海湿地的影响及其保护对策[J]. 湿地科学, 2007, 5(3): 282~288.
[4]	沈德中. 污染环境生物修复 [M]. 北京:化学工业出版社, 2002.
[5]	李海明, 马杰, 顾晓明,等. 渤海滩涂湿地沉积物胶体对石油污染物的吸附特征[J]. 湿地科学, 2008, 6(2): 218~222.
[6]	张海荣, 李培军, 孙铁珩, 等. 四种石油污染土壤生物修复技术研究[J]. 农业环境保护, 2001, 20(2): 78~80.
[7]	陆秀君, 郭书海, 孙清, 等. 石油污染土壤的修复技术研究现状及展望[J]. 沈阳农业大学学报,2003, 34(1): 63~67.
[8]	蔺昕, 李培军, 台培东, 等. 石油污染土壤植物-微生物修复研究进展[J]. 生态学杂志, 2006, 25(1): 93~100.
[9]	詹研. 中国土壤石油污染的危害及治理对策[J]. 环境污染与防治, 2008, 30(3): 91~94.
[10]	Salt D E, Blaylock M, Kumar N P B A, et al. Phytoremediation - a novel strategy for the removal of toxic metals from the environment using plants[J]. Bio-Technology, 1995, 13(5): 468~474.
[11]	宋晓林,吕宪国. 中国退化河口湿地生态恢复研究进展[J]. 湿地科学, 2009, 7(4): 379~384.
[12]	Wang Y X,Oyaizu H.Evaluation of the phytoremediation potential of four plant species for dibenzofuran-contaminated soil[J]. Journal of Hazardous Materials, 2009, 168(2-3): 760-764.
[13]	Lin Q X, Mendelssohn I A, Suidan M T, et al.The dose-response relationship between No. 2 fuel oil and the growth of the salt marsh grass, Spartina alterniflora[J]. Marine Pollution Bulletin, 2002, 44(9): 897-902.
[14]	Huang X D, El-Alawi Y, Penrose D M, et al.Responses of three grass species to creosote during phytoremediation[J]. Environmental Pollution, 2004, 130(3): 453-463.
[15]	Kaimi E, Mukaidani T, Miyoshi S, et al.Ryegrass enhancement of biodegradation in diesel-contaminated soil[J]. Environmental and Experimental Botany, 2006, 55(1-2): 110-119.
[16]	Tang J C, Wang R G, Niu X W, et al.Enhancement of soil petroleum remediation by using a combination of ryegrass (Lolium perenne) and different microorganisms[J]. Soil & Tillage Research, 2010, 110(1): 87-93.
[17]	Palmroth M R T, Pichtel J,Puhakka J A. Phytoremediation of subarctic soil contaminated with diesel fuel[J]. Bioresource Technology, 2002, 84(3): 221-228.
[18]	徐崇彦, 刘宪斌, 刘占广, 等. 翅碱蓬对石油烃污染的海岸带修复的初步研究[J]. 安全与环境学报, 2007, 7(1): 37~39.
[19]	Zhang Z N, Zhou Q X, Peng,S W,et al.Remediation of petroleum contaminated soils by joint action of Pharbitis nil L. and its microbial community[J]. Science of the Total Environment, 2010, 408(22): 5600-5605.
[20]	张宪政. 植物叶绿素含量测定-丙酮乙醇混合液法[J]. 辽宁农业科学, 1986, (3): 26~28.
[21]	王雪峰, 陈桂珠,徐夏玲. 白骨壤对石油污染的生理生态响应[J]. 生态学报, 2005, 25(5): 1095~1100.
[22]	李玫,陈桂珠. 含油废水对秋茄幼苗的几个生理生态指标的影响[J]. 生态学报, 2000, 20(3): 528~532.
[23]	Lin Q X,Mendelssohn I A.Potential of restoration and phytoremediation with Juncus roemerianus for diesel-contaminated coastal wetlands[J]. Ecological Engineering, 2009, 35(1): 85-91.
[24]	刘亚云, 孙红斌, 陈桂珠, 等. 秋茄(Kandelia candel)幼苗对多氯联苯污染的生理生态响应[J]. 生态学报, 2007, 27(2): 746~754.
[25]	李秀珍, 李彬. 重金属对植物生长发育及品质的影响[J]. 安徽农业科学, 2008, 36(14): 5742~5746.
[26]	籍国东, 孙铁珩, 隋欣, 等. 落地原油对芦苇湿地生态工程净化系统影响[J]. 生态学报, 2002, 22(5): 649~654.
[27]	王学征, 韩文灏, 于广建. 盐分胁迫对番茄幼苗生理生化指标影响的研究[J]. 北方园艺, 2004, (3): 48~49.
[28]	肖强, 郑海雷, 陈瑶, 等. 盐度对互花米草生长及脯氨酸、可溶性糖和蛋白质含量的影响[J]. 生态学杂志, 2005, 24(4): 373~376.
[29]	陈明林, 王友保. 水分胁迫下外来种铜锤草和本地种酢浆草的生理指标比较研究[J]. 草业科学, 2008, 17(6): 52~59.
[30]	刘祖祺, 张石诚. 植物抗性生理学 [M]. 北京: 中国农业出版社, 1994.
[31]	张凤琴, 王友绍, 董俊德, 等. 重金属污水对木榄幼苗几种保护酶及膜脂质过氧化作用的影响[J]. 热带海洋学报, 2006, 25(2): 66~70.
[32]	吴家燕, 夏增禄, 巴音,等. 土壤重金属污染的酶学诊断——紫色土中的的镉、铜、铅、砷对水稻根系过氧化物酶的影响[J]. 环境科学学报, 1990, 10(1): 73~77.
[33]	郑福丽, 江丽华, 刘兆辉, 等. 石油污染物对油菜产量及生理指标的影响[J]. 中国农学通报, 2009, 25(19): 279~282.

试验处理	30 d 株高（cm）分蘖数		45 d 株高（cm）分蘖数（个）
对照组	15.20±2.944	6.0±0.816	28.81±1.643	8.3±0.957
低污染组	10.24±2.292^*	7.0±1.414	22.69±3.439^*	8.3±0.957
高污染组	10.06±2.278	5.3±0.957^*	21.24±3.497	7.8±1.893

试验处理	30 d 株高（cm）叶片数（片）		45 d 株高（cm）分枝数（个）
对照组	8.11±0.318	21.0±3.741	25.60±0.835	8.5±1.000
低污染组	3.88±0.128^**	9.2±0.500^**	9.82±0.371^**	3.3±0.500^**
高污染组	2.89±0.159^**	6.5±1.00	5.84±0.343^**	0.5±0.577^**

试验处理	叶绿素总量	叶绿素a	叶绿素b	叶绿素a/b
对照组	4.566±0.609	3.506±0.462	1.061±0.148	3.307±0.053
低污染组	2.760±0.396^**	2.141±0.299^**	0.619±0.097^**	3.466±0.064^**
高污染组	2.966±0.568	2.292±0.437	0.674±0.130	3.399±0.025

试验处理	叶绿素总量	叶绿素a	叶绿素b	叶绿素a/b
对照组	0.637±0.027	0.463±0.029	0.175±0.009	2.659±0.241
低污染组	0.586±0.036^*	0.434±0.028	0.152±0.008^**	2.849±0.471
高污染组	0.362±0.030^**	0.262±0.026^**	0.100±0.006^**	2.705±0.193

黄河三角洲石油污染对湿地芦苇和碱蓬幼苗生长影响的模拟研究

Simulative Study on Effects of Petroleum Contamination on Seedling Growth of Phragmites australis and Suaeda salsa in Coastal Wetland of Yellow River Delta

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