Abstract: Biodiversity is very important natural resource. It is our human beings’ material base for survival. The protection of biodiversity has been paid more and more attention to. How to protect biodiversity is a key research issue to ecologists. In this paper, we discussed protecting biodiversity from a landscape ecology prospective. Four landscape factors, patch, edge, corridor and disturbance are selected to discuss their relations with biodiversity. It has a great significance to understand and apply these relations. It will provide us with a powerful tool for reserve designing, species managing and land use planning.