Abstract: Recently, along with the acceleration of urbanization, motorization, the urban transportation problem has been deteriorated increasingly in China. So the study on the mutual relationship between urban transport system and land use has aroused the attention of the Chinese scholars and specialists more and more. As one of the rapid-growing mega-cities in South China, Guangzhou now has stepped into a transformation period, both in industried structure and social system. Urban land use is in typical high density. Urban transport system began to change from the traditional foot-and-bicycle pattern to a motorized pattern. Land shortage and traffic congestion have brought great tension. A case study in Guangzhou, revedling the specific impacts on land use of urban transport system and its mechanism, will surely benefit the solution of urban transport problems in Chinese cities, and then promote their sustainability. On the basis of literature review about the impacts on urban land use of transport system, quantitative methods and special analysis technology like GIS and RS are cautiously adopted besides traditional methods, this paper studies the impacts on urban land use of transport system in Guangzhou, in terms of documents, data and on-the-spot investigation. Firstly, it discusses the evolvement of urban space pattern affected by the development of transport system of Guangzhou. With the impacts of urban transport system, the urban space pattern of Guangzhou has experienced the evolvement from compacted city to sparsely distributed group city, belt-and-group city and multi-core, half-networked city at last. Then, the simulative effects on land use development of urban transport system construction are examined. Through GIS and RS analyses methods, it gives an all-around research on the effects of urban transport system on urban land use and land exploitation. And it is found that there is an intensively simulative and attractive effect on urban land use of urban transport construction. Thirdly, the impacts on urban land use price of urban transport system characters are explained. Through the quantitative and GIS methods, it is discovered that the transport system has a profound impact on commercial and residential land use price in Guangzhou. Fourthly, the impacts of urban transport system on urban land use layout are illustrated. It is founded that there is profound impact on commercial, residential, industry land use layout in Guangzhou too. Finally, a brief conclusion is drawn on. We hold the opinion that the study on the impacts of urban transport system on urban land use will benefit to the searching for the root of urban transport problems and the solving measures of transport problems in China.