Abstract: It is significant for the urban traffic administration to obtain the dynamic travel regularity of colony vehicles with the same or similar route. Unfortunately, there is no such an efficient method yet. To solve the issue,considering the traffic route linked by nodes including tiny traffic zones and traffic intersections, the typical colony route theory based on Spatial Analysis is proposed to describe the travel regularity of colony vehicles. Furthermore an algorithm to acquire the typical colony route is researched based on dynamic Origin-Destination (OD) analysis. Firstly, by means of the statistic of spatial traffic flow parameters collected by GPS or shooting-identifying device deployed at the road intersections, the hot traffic spots can be hunted. Accordingly, the candidate routes can be traced by calculating the occupancy proportion of the routes concerning these hot traffic spots. Secondly, resorting to fuzzy clustering of traffic intersections during the analyzing course of the similar candidate routes, the tiny zone nodes and the colony-route set can be obtained. At last, the typical colony route containing traffic intersections and tiny traffic zones can be generated by sorting the colony-route set. Finally, based on the proposed typical colony route theory coupled with the algorithms, the software is developed to hunt for typical colony route automatically. Take the experimental traffic grid in Hangzhou City as an example, two typical colony routes is successfully acquired. According to the result of manual static OD investigating and manual statistical surveying, both typical colony routes are in accordance with the real travel regularity. Comparing with the previous manual methods, the proposed solution does not only demonstrate better performance in efficiency but also be able to reflect the dynamic route regularity of vehicle colony travel just in good time at urban district. The research could facilitate the urban traffic administration to adopt proper countermeasure to eliminate traffic jam and traffic trip pressure. Referring to the dynamic travel regularity of colony vehicles, the urban traffic administration could publish the traffic navigation message concerning the whole typical colony route at the route’s key intersection, and improve the deployment of the bus station or the route plan of urban public bus.